PXD036148-1

PXD036148 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of the Mitochondria of Menstrual Stem Cells and Ovarian Cancer Cells
Description	Mitochondria transplantation is a hot spot in cell-free therapy. Menstrual stem cells (MenSCs) are potential donor cells to provide foreign mitochondria. This study aims to investigate the possible effects of MenSC-derived mitochondria on ovarian cancer from the perspective of protein expression profiling. MenSCs were harvested from menstrual blood. The mitochondria were isolated from MenSCs and ovarian cancer cell line-SKOV3. A label-free proteomics and analysis were performed by comparing the protein expression in the mitochondria of MenSCs and SKOV3 cells. The differentially expressed proteins with fold change higher than 2 were analyzed by Gene Ontology, KEGG pathway and protein domain enrichment analysis, protein interaction networks and PRM analysis. In total, 592 proteins which were up-expressed in the mitochondria of MenSCs were taken to be analyzed. Functional enrichment analysis revealed these proteins were enriched in metabolism-related pathway entries including oxidative phosphorylation (OXPHOS) pathway. Parallel Reaction Monitoring (PRM) analysis confirmed that 4 out of 6 candidate proteins on OXPHOS pathway afforded similar trends. The protein domain enrichment analysis showed that the domains such as thioredoxin domain were significantly enriched. Based on these findings, we believed that the mitochondria from MenSCs might work to enhance the progression of ovarian cancer, which was probably mediated by the enrichment of OXPHOS-related metabolic pathways.
HostingRepository	PRIDE
AnnounceDate	2022-10-12
AnnouncementXML	Submission_2022-10-12_09:37:59.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wenzhong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	MaxQuant

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-18 07:54:46	ID requested
⏵ 1	2022-10-12 09:37:59	announced

Dataset with its publication pending

submitter keyword: Energy metabolism, Menstrual stem cell, Mitochondria transplantation, OXPHOS, Proteomics

XiuhuiChen
contact affiliation	Department of obstetrics and gynecology. Second affiliated hospital of Harbin Medical University. Harbin, Heilongjiang, China.
contact email	106942878@qq.com
lab head
wenzhong
contact affiliation	The 2nd affiliated hospital of Harbin medical university
contact email	drzhong96@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036148
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of the Mitochondria of Menstrual Stem Cells and Ovarian Cancer Cells