PXD036106-2

PXD036106 is an original dataset announced via ProteomeXchange.

Title	Extended DNA threading through a dual-engine motor module in the activating signal co-integrator complex
Description	Activating signal co-integrator complex (ASCC) supports diverse genome maintenance and gene expression processes. Its ASCC3 subunit is an unconventional nucleic acid helicase, harboring tandem Ski2-like NTPase/helicase cassettes crucial for ASCC functions. Presently, the molecular mechanisms underlying ASCC3 helicase activity and regulation remain unresolved. Here, we present cryogenic electron microscopy, DNA-protein cross-linking/mass spectrometry as well as in vitro and cellular functional analyses of the ASCC3-ASC1/TRIP4 sub-module of ASCC. Unlike the related spliceosomal SNRNP200 RNA helicase, ASCC3 can thread substrates through both helicase cassettes. ASC1 docks on ASCC3 via a zinc finger domain and stimulates the helicase by positioning a C-terminal ASC1-homology domain next to the C-terminal helicase cassette of ASCC3, likely assisting the DNA exit. ASC1 binds ASCC3 mutually exclusively with the dealkylase, ALKBH3, directing ASCC for specific ASCC-dependent processes. Our findings define ASCC3-ASC1/TRIP4 as a tunable motor module of ASCC that encompasses two cooperating ATPase/helicase units functionally expanded by ASC1/TRIP4.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:42:33.589.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandar Chernev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 13:51:21	ID requested
1	2023-03-06 09:45:49	announced
⏵ 2	2024-10-22 05:42:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Protein-DNA cross-linking

Henning Urlaub
contact affiliation	Max-Planck-Institute for Multidisciplinary Sciences Bioanalytical Mass Spectrometry Group University Medical Center Goettingen Bioanalytics
contact email	henning.urlaub@mpinat.mpg.de
lab head
Aleksandar Chernev
contact affiliation	MPIbpc
contact email	aleksandar.chernev@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036106

PRIDE project URI

• PRIDE
  1. PXD036106
     1. Label: PRIDE project
     2. Name: Extended DNA threading through a dual-engine motor module in the activating signal co-integrator complex