PXD036093-1

PXD036093 is an original dataset announced via ProteomeXchange.

Title	Proteomics Reveals the Potential Mechanism of Tanshinone IIA Promoting the Ex Vivo Expansion of Human Bone Marrow Mesenchymal Stem Cells.
Description	Bone marrow mesenchymal stem cells (BMSCs) is a promising cell type for tissue engineering, however, the application of BMSCs are largely hampered for the limited number harvested from bone marrow cells. The methods or strategies that focused on promoting the capacity of BMSCs expansion ex vivo become more and more important. Tanshinone IIA (Tan IIA), the main active components of Danshen, has been found to promote BMSCs proliferation, but the underlying mechanism is still unclear. This present work employed biological techniques combined with quantitative proteome analysis to explore the effect and underlying mechanism of Tan IIA on the ex vivo expansion capacity of BMSCs from human. The results showed that a total of 84 differentially expressed proteins were annotated and among them 51 upregulated proteins and 33 downregulated proteins were identified. Besides, Tan IIA could promote hBMSCs proliferation by regulating the progression of S phase via increasing the release of fibroblast growth factor 2 (FGF2), FGF-mediated PI3K/AKT signaling pathways may play an important role in Tan IIA’s effect on hBMSCs expansion. The results of this study may provide further evidence about the potential of Tan IIA to be used for the research of hBMSCs therapies.
HostingRepository	PRIDE
AnnounceDate	2022-11-28
AnnouncementXML	Submission_2022-11-28_07:50:05.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PeiYuan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 13:31:10	ID requested
⏵ 1	2022-11-28 07:50:06	announced

Dataset with its publication pending

submitter keyword: bone marrow mesenchymal stem cells

Tanshinone IIA

proteomics

expansion

fibroblast growth factor 2

autocrine.

XunLi
contact affiliation	The First Clinical Medical College, Lanzhou University; Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province; The Medical Frontier Innovation Research Center, The First Hospital of Lanzhou University; The Department of General Surgery, The First Hospital of Lanzhou University; Hepatopancreatobiliary Surgery Institute of Gansu Province, Lanzhou 730000, Gansu Province, China.
contact email	yuanp19@lzu.edu.cn
lab head
PeiYuan
contact affiliation	The First Clinical Medical College of Lanzhou University
contact email	yuanp19@lzu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD036093

PRIDE project URI

• PRIDE
  1. PXD036093
     1. Label: PRIDE project
     2. Name: Proteomics Reveals the Potential Mechanism of Tanshinone IIA Promoting the Ex Vivo Expansion of Human Bone Marrow Mesenchymal Stem Cells.