PXD036088-1

PXD036088 is an original dataset announced via ProteomeXchange.

Title	Dynamic acylome reveals metabolite driven modifications in Syntrophomonas wolfei
Description	Syntrophomonas wolfei is an anaerobic syntrophic microbe that degrades short-chain fatty acids to acetate, hydrogen, and/or formate. This thermodynamically unfavorable process proceeds through a series of reactive acyl-Coenzyme A species (RACS). In other prokaryotic and eukaryotic systems, the production of intrinsically reactive metabolites correlates with acyl-lysine modifications, which have been shown to play a significant role in metabolic processes. Analogous studies with syntrophic bacteria, however, are relatively unexplored and we hypothesize that highly abundant acylations could exist in S. wolfei proteins, corresponding to the RACS derived from degrading fatty acids. Here, by mass spectrometry-based proteomics (LC-MS/MS), we characterize and compare acylome profiles of two S. wolfei subspecies grown on different carbon substrates. Because modified S. wolfei proteins are sufficiently abundant to analyze post-translational modifications (PTMs) without antibody enrichment, we could identify types of acylations comprehensively, observing six types (acetyl-, butyryl-, 3-hydroxybutyryl-, crotonyl-, valeryl-, and hexanyl-lysine), two of which have not been reported in any system previously. All of the acyl-PTMs identified correspond directly to RACS in fatty acid degradation pathways. A total of 369 sites of modification were identified on 237 proteins. Structural studies and in vitro acylation assays of a heavily modified enzyme, acetyl-CoA transferase, provided insight on the potential impact of these acyl-protein modifications. The extensive changes in acylation-type, abundance, and modification sites with carbon substrate suggest that protein acylation by RACS may be an important regulator of syntrophy.
HostingRepository	PanoramaPublic
AnnounceDate	2022-10-13
AnnouncementXML	Submission_2022-10-13_10:20:25.876.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Janine Fu
SpeciesList	scientific name: Syntrophomonas wolfei; NCBI TaxID: 863;
ModificationList	Acetyl; Butyryl; Carbamidomethyl; Oxidation
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-16 13:25:30	ID requested
⏵ 1	2022-10-13 10:20:26	announced

no publication

submitter keyword: lysine acylation, post-translational modifications, Syntrophs, Syntrophomonas wolfei

Joseph A. Loo
contact affiliation	University of California, Los Angeles
contact email	jloo@chem.ucla.edu
lab head
Janine Fu
contact affiliation	University of California, Los Angeles
contact email	janinefu129@ucla.edu
dataset submitter

Panorama Public dataset URI