PXD036057-1
PXD036057 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC |
| Description | Polycomb (PcG) silencing is crucial for development, but how targets are specified remains incompletely understood. The cold-induced Polycomb Repressive Complex 2 (PRC2) silencing of Arabidopsis thaliana FLOWERING LOCUS C (FLC) provides an excellent system to elucidate PcG regulation. Association of the DNA binding protein VAL1 to the PcG nucleationregion at FLC is an important step. VAL1 interacts with APOPTOSIS AND SPLICING ASSOCIATED PROTEIN (ASAP) complex and PRC1. Here, we show that ASAP and PRC1 are necessary for co-transcriptional repression and chromatin regulation during FLC silencing. ASAP mutants affect FLC transcription in warm conditions, but the rate of FLC silencing in the cold is unaffected. PRC1-mediated H2Aub accumulates at FLC nucleation region during cold, but unlike the PRC2-delivered H3K27me3 does not spread across the locus. H2Aub thus marks the transition to epigenetic silencing, while H3K27me3 is necessary for long-term epigenetic memory. Overall, our work highlights the importance of VAL1 as an assembly platform co-ordinating the co-transcriptional repression and chromatin regulation necessary for the epigenetic silencing of FLC. |
| HostingRepository | iProX |
| AnnounceDate | 2022-08-15 |
| AnnouncementXML | Submission_2022-08-15_05:58:12.325.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tiancong Lu |
| SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
| ModificationList | acetylated residue; methionine oxidation with neutral loss of 64 Da; S-carboxamidomethyl-L-cysteine |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-08-15 05:58:05 | ID requested | |
| ⏵ 1 | 2022-08-15 05:58:12 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: co-transcriptional repression, VAL1, FLC regulation |
Contact List
| Caroline Dean | |
|---|---|
| contact affiliation | John Innes Centre |
| contact email | caroline.dean@jic.ac.uk |
| lab head | |
| Tiancong Lu | |
| contact affiliation | John Innes Centre |
| contact email | proteomelandscape@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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