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PXD036049-1

PXD036049 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics and phosphoproteomics analyse the effect of ocnus on Drosophila spermatogenesis
DescriptionFor phophoproteomics, the nosGal4>w- (control) samples 1, 2, and 3 were labeled with iTRAQ tags 118, 119, and 121, respectively. Samples of nosGal4>ocn-hp 1, 2, and 3 were labeled with iTRAQ tags 115, 116, and 117, respectively. The labeled peptides were desalted and concentrated in Sep-Pack C18 Cartridges (Waters, Milford, MA, USA). Then, iTRAQ-labeled peptides were added into the freshly prepared TiO2 beads at peptides-to-beads ratio of 1 : 4 for phosphopeptide enrichment. The peptide-beads’ slurry was shaken on a rotator at 37 °C for 1 h and then centrifuged for 30 s and then washed. Phosphopeptides were finally eluted with elution buffer for further analysis.
HostingRepositoryiProX
AnnounceDate2022-08-13
AnnouncementXMLSubmission_2022-08-14_19:20:43.491.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYa Zheng
SpeciesList scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-14 19:20:18ID requested
12022-08-14 19:20:43announced
22023-05-29 20:00:07announced2023-05-30: Update publication information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, phosphoproteomics, ocnus, Drosophila spermatogenesis
Contact List
Yufeng Wang
contact affiliationCentral China Normal University
contact emailyfengw@ccnu.edu.cn
lab head
Ya Zheng
contact affiliationCentral China Normal University
contact emailzhengyahb@mail.ccnu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI