PXD036049-1
PXD036049 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics and phosphoproteomics analyse the effect of ocnus on Drosophila spermatogenesis |
Description | For phophoproteomics, the nosGal4>w- (control) samples 1, 2, and 3 were labeled with iTRAQ tags 118, 119, and 121, respectively. Samples of nosGal4>ocn-hp 1, 2, and 3 were labeled with iTRAQ tags 115, 116, and 117, respectively. The labeled peptides were desalted and concentrated in Sep-Pack C18 Cartridges (Waters, Milford, MA, USA). Then, iTRAQ-labeled peptides were added into the freshly prepared TiO2 beads at peptides-to-beads ratio of 1 : 4 for phosphopeptide enrichment. The peptide-beads’ slurry was shaken on a rotator at 37 °C for 1 h and then centrifuged for 30 s and then washed. Phosphopeptides were finally eluted with elution buffer for further analysis. |
HostingRepository | iProX |
AnnounceDate | 2022-08-13 |
AnnouncementXML | Submission_2022-08-14_19:20:43.491.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ya Zheng |
SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-08-14 19:20:18 | ID requested | |
⏵ 1 | 2022-08-14 19:20:43 | announced | |
2 | 2023-05-29 20:00:07 | announced | 2023-05-30: Update publication information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, phosphoproteomics, ocnus, Drosophila spermatogenesis |
Contact List
Yufeng Wang | |
---|---|
contact affiliation | Central China Normal University |
contact email | yfengw@ccnu.edu.cn |
lab head | |
Ya Zheng | |
contact affiliation | Central China Normal University |
contact email | zhengyahb@mail.ccnu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |