PXD036049-1

PXD036049 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics and phosphoproteomics analyse the effect of ocnus on Drosophila spermatogenesis
Description	For phophoproteomics, the nosGal4>w- (control) samples 1, 2, and 3 were labeled with iTRAQ tags 118, 119, and 121, respectively. Samples of nosGal4>ocn-hp 1, 2, and 3 were labeled with iTRAQ tags 115, 116, and 117, respectively. The labeled peptides were desalted and concentrated in Sep-Pack C18 Cartridges (Waters, Milford, MA, USA). Then, iTRAQ-labeled peptides were added into the freshly prepared TiO2 beads at peptides-to-beads ratio of 1 : 4 for phosphopeptide enrichment. The peptide-beads’ slurry was shaken on a rotator at 37 °C for 1 h and then centrifuged for 30 s and then washed. Phosphopeptides were finally eluted with elution buffer for further analysis.
HostingRepository	iProX
AnnounceDate	2022-08-13
AnnouncementXML	Submission_2022-08-14_19:20:43.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Zheng
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-14 19:20:18	ID requested
⏵ 1	2022-08-14 19:20:43	announced
2	2023-05-29 20:00:07	announced	2023-05-30: Update publication information.

Dataset with its publication pending

submitter keyword: proteomics, phosphoproteomics, ocnus, Drosophila spermatogenesis

Yufeng Wang
contact affiliation	Central China Normal University
contact email	yfengw@ccnu.edu.cn
lab head
Ya Zheng
contact affiliation	Central China Normal University
contact email	zhengyahb@mail.ccnu.edu.cn
dataset submitter

iProX dataset URI