PXD035996-1

PXD035996 is an original dataset announced via ProteomeXchange.

Title	KCNQ1OT1 promotes genome-wide transposon repression by guiding RNA-DNA triplexes and HP1 binding
Description	Transposon (de)repression and heterochromatin reorganization are dynamically regulated during cell fate determination and are hallmarks of cellular senescence. However, whether they are sequence specifically regulated remains unknown. Here, we uncover the KCNQ1OT1 lncRNA, by sequence-specific Hoogsteen base-pairing with double-stranded genomic DNA via its repeat-rich region and binding to heterochromatin protein HP1⍺, guides, induces and maintains epigenetic silencing at specific repetitive DNA elements. Repressing KCNQ1OT1 or deleting its repeat-rich region reduces DNA methylation and H3K9me3 on KCNQ1OT1 targeted transponsons. Engineering a fusion KCNQ1OT1 with an ectopically targeting guiding triplex sequence induces de novo DNA methylation at the target site. Phenotypically, repressing KCNQ1OT1 induces senescence associated heterochromatin foci, transposon activation and retrotransposition, and cellular senescence, demonstrating an essential role of KCNQ1OT1 to safeguard against genome instability and senescence.
HostingRepository	PRIDE
AnnounceDate	2022-09-05
AnnouncementXML	Submission_2022-09-04_19:53:17.050.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035996
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jiyang LI
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-12 02:20:10	ID requested
⏵ 1	2022-09-04 19:53:17	announced

Dataset with its publication pending

submitter keyword: KCNQ1OT1, heterochromatin, transposon, RNA-dsDNA triplex, senescence

Jing-Dong J. Han
contact affiliation	Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Center for Quantitative Biology (CQB), Peking University; Beijing 100871, China
contact email	jackie.han@pku.edu.cn
lab head
Jiyang LI
contact affiliation	CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; 320 Yue Yang Road, Shanghai, 200031, China University of Chinese Academy of Sciences; Beijing 100049, China School of Life Science and Technology, ShanghaiTech University; Shanghai, 201210, China
contact email	meng_ljy@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD035996

PRIDE project URI

• PRIDE
  1. PXD035996
     1. Label: PRIDE project
     2. Name: KCNQ1OT1 promotes genome-wide transposon repression by guiding RNA-DNA triplexes and HP1 binding