PXD035973-1

PXD035973 is an original dataset announced via ProteomeXchange.

Title	ELTD1 is present in extracellular vesicles derived from endothelial cells as a cleaved extracellular domain which induces in vivo angiogenesis
Description	ELTD1/ADGRL4 is an adhesion GPCR with an important role in angiogenesis. We recently identified a role for ELTD1 in wound repair and inflammation. Activation of ELTD1 in endothelial cells results in a type II EMT to myofibroblast-like cells that have enhanced angiogenic ability. Furthermore, expression of Eltd1 in murine breast cancer cells increases tumour growth by increasing blood vessel size and perfusion and by creating an immunosuppressive microenvironment. We thereforeAs extracellular vesicles (EVs) are known to be involved in vascular development, growth and maturation we investigated the composition and functional effects of the extracellular vesicles (EVs) isolated from ELTD1 expressexpressing cells to elucidate their role in these processes. A highly glycosylated form of the extracellular domain of ELTD1 is readily incorporated into EVs. Using mass spectrometry-based proteomics we identified proteins that are enriched in ELTD1-EVs and are involved in haemostasis and immune responses. ELTD1 enriched EVs were pro-angiogenic in vivo and in vitro and the presence of the extracellular domain alone induced endothelial sprouting. In endothelial cells experiencing laminar flow, ELTD1 levels were reduced in the EVs when compared to quiescent cells, showing a relationship between ELTD1 and the activation state of the endothelium. Using FACS, we detected a significant increase in vesicular ELTD1 in the plasma of patients with preeclampsia, a condition characterized by endothelial dysfunction. These data confirm a role for ELTD1 in wound repair and inflammation and reveal its potential as a biomarker of vessel dysfunction.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_11:29:02.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Svenja Hester
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue; iodoacetamide derivatized residue
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-11 02:38:29	ID requested
⏵ 1	2022-08-11 11:29:02	announced
2	2023-11-14 08:31:52	announced	2023-11-14: Updated project metadata.

10.1002/JEX2.52;

submitter keyword: angiogenesis, ELTD1, wound repair, inflammation,

Adrian L. Harris1
contact affiliation	Department of Oncology University of Oxford
contact email	adrian.harris@oncology.ox.ac.uk
lab head
Svenja Hester
contact affiliation	Nuffield Department of Medicine
contact email	svenja.hester@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035973
     1. Label: PRIDE project
     2. Name: ELTD1 is present in extracellular vesicles derived from endothelial cells as a cleaved extracellular domain which induces in vivo angiogenesis