PXD035945 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of prolyl 4-hydroxylation of P4ha1 KO MEFs and P4ha2 KO mouse kidneys |
Description | We established a mouse embryonic fibroblast (MEF) cell line derived from mice that have a genetic ablation of of P4ha1. Type I collagen produced by these cells in presence of ascorbate was collected and partially purified by precipitation. Site specific hydroxylation analysis was performed using mass spectrometry. In another setup, type IV collagen from kidneys of P4ha2-/- mice was partially purified using pepsin digestion in acetic acid. Gel bands corresponding type IV collagens were cut and analyzed by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-15 |
AnnouncementXML | Submission_2023-12-15_03:23:16.193.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pekka Rappu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | O5-galactosyl-L-hydroxylysine; O5-glucosylgalactosyl-L-hydroxylysine; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-10 09:21:03 | ID requested | |
⏵ 1 | 2023-12-15 03:23:16 | announced | |
Publication List
Keyword List
submitter keyword: mouse, prolyl 4-hydroxylation, collagen |
Contact List
Jyrki Heino |
contact affiliation | Department of Life Technologies, University of Turku, Turku, Finland |
contact email | jyrki.heino@utu.fi |
lab head | |
Pekka Rappu |
contact affiliation | University of Turku |
contact email | pekka.rappu@utu.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035945
- Label: PRIDE project
- Name: Analysis of prolyl 4-hydroxylation of P4ha1 KO MEFs and P4ha2 KO mouse kidneys