PXD035909-2

PXD035909 is an original dataset announced via ProteomeXchange.

Title	Spermidine is essential for fasting-mediated autophagy and longevity
Description	Caloric restriction and intermittent fasting prolong the lifespan and healthspan of model organisms and improve human health 1. The natural polyamine spermidine has been linked to autophagy regulation, geroprotection and reduced incidence of cardiovascular and neurodegenerative diseases across species borders 2. Here, we report that spermidine levels increase upon acute fasting in yeast, flies, mice and healthy humans. Genetic or pharmacological blockade of endogenous spermidine synthesis reduced fasting-induced autophagy in yeast, worms and human cells. Furthermore, perturbing the polyamine pathway in vivo abrogated the lifespan-extending, cardioprotective and antiarthritic effects of intermittent fasting. Mechanistically, spermidine mediated these effects via hypusination of the autophagy regulator eIF5A. In sum, the polyamine-hypusination axis thus emerges as a bona fide and phylogenetically conserved metabolic control hub for longevity and autophagy induction.
HostingRepository	PRIDE
AnnounceDate	2024-08-08
AnnouncementXML	Submission_2024-08-08_07:54:38.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Masser
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-09 02:12:56	ID requested
1	2024-04-30 13:34:52	announced
⏵ 2	2024-08-08 07:54:38	announced	2024-08-08: Updated project metadata.

10.1038/S41556-024-01468-X;

submitter keyword: autophagy, NanoLC-MS/MS,spermidin, fasting, yeast, human, lifespan, eIF5A hypusination, mouse, C.elegans

Ulrich Stelzl
contact affiliation	Institute of Pharmaceutical Sciences, Pharmaceutical Chemistry, University of Graz, Austria
contact email	ulrich.stelzl@uni-graz.at
lab head
Sarah Masser
contact affiliation	University of Graz, Institute of Pharmaceutical Sciences
contact email	sarah.masser@uni-graz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD035909

PRIDE project URI

• PRIDE
  1. PXD035909
     1. Label: PRIDE project
     2. Name: Spermidine is essential for fasting-mediated autophagy and longevity