PXD035877-1

PXD035877 is an original dataset announced via ProteomeXchange.

Title	A method for targeting a specified segment of DNA to a bacterial microorganelle
Description	Our work presents a protocol for the encapsulation and subsequent affinity isolation of the proteinaceous compartment carrying a DNA segment. To this end, we have repurposed the microcompartment of Citrobacter freundii for propaneadiol utilisation (Pdu) and constructed a LacI transcription repressor that allows the assembly of a synthetic organelle containing the LacI-DNA complex. High-throughput sequencing of extracted DNA from the affinity-isolated MCPs shows that our strategy results in leads to packaging of a DNA segment carrying multiple LacI binding sites, but not the flanking regions.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_02:14:59.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matej Butala
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	alkylated residue
Instrument	solariX

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-08 02:20:03	ID requested
⏵ 1	2022-08-11 02:15:00	announced
2	2023-11-14 08:57:37	announced	2023-11-14: Updated project metadata.

10.1093/NAR/GKAC714;

submitter keyword: Escherichia coli, Citrobacter freundii, propanediol utilisation (Pdu) microcompartment, repressor LacI

Matej Butala
contact affiliation	University of Ljubljana, Biotechnical Faculty, Department of biology, Chair of biochemistry, Večna pot 111, 1000 Ljubljana
contact email	matej.butala@bf.uni-lj.si
lab head
Matej Butala
contact affiliation	University of Ljubljana, Biotechnical Faculty, Večna pot 111, 1000 Ljubljana
contact email	matej.butala@bf.uni-lj.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD035877

PRIDE project URI

• PRIDE
  1. PXD035877
     1. Label: PRIDE project
     2. Name: A method for targeting a specified segment of DNA to a bacterial microorganelle