PXD035866-3
PXD035866 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted protein degradation reveals Pol II heterogeneity and functional diversity |
Description | RNA polymerase II (Pol II) subunits are thought to be involved in various transcription-associated processes, but it is unclear whether they play different regulatory roles in modulating gene expression. Here, we performed nascent and mature transcript sequencing after the acute degradation of 12 mammalian Pol II subunits and profiled their genomic binding sites and protein interactomes to dissect their molecular functions. We found that Pol II subunits contribute differently to Pol II cellular localization and transcription process and preferentially regulate RNA processing (such as RNA splicing and 3’ end maturation). Genes sensitive to the depletion of different Pol II subunits tend to be involved in diverse biological functions and show different RNA half-lives. Sequences, associated protein factors, and RNA structures are correlated with Pol II subunit-mediated differential gene expression. These findings collectively suggest that the heterogeneity of Pol II and different genes appear to depend on some of the subunits. |
HostingRepository | iProX |
AnnounceDate | 2022-08-09 |
AnnouncementXML | Submission_2023-12-21_20:44:28.277.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xiong Ji |
SpeciesList | scientific name: Mus |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-08-08 00:08:52 | ID requested | |
1 | 2022-08-08 00:09:13 | announced | |
2 | 2022-08-08 19:59:14 | announced | 2022-08-09: Update information. |
⏵ 3 | 2023-12-21 20:44:29 | announced | 2022-08-09: Update information. 2023-12-22: Update publication information. |
Publication List
Li Y, Huang J, Zhu J, Bao L, Wang H, Jiang Y, Tian K, Wang R, Zheng H, Duan W, Lai W, Yi X, Zhu Y, Guo T, Ji X, Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity. Mol Cell, 82(20):3943-3959.e11(2022) [pubmed] |
Keyword List
submitter keyword: Transcription, Pol II subunits, ChIP-MS |
Contact List
Xiong Ji | |
---|---|
contact affiliation | Peking University |
contact email | xiongji@pku.edu.cn |
lab head | |
Xiong Ji | |
contact affiliation | Peking University |
contact email | xiongji@pku.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |