PXD035761-1

PXD035761 is an original dataset announced via ProteomeXchange.

Title	Identification of putative reader proteins of RNA 5-methylcytosine and its derivatives in Caenorhabditis elegans
Description	Methylation of carbon-5 of cytosines (m5C) is a conserved post-transcriptional nucleotide modification of RNA with widespread distribution across organisms. m5C has been shown to participate in mRNA transport and maintain mRNA stability through its recognition by the reader proteins ALYREF and YBX1, respectively. We recently showed that m5C is required for Caenorhabditis elegans development and fertility under heat stress. To contribute to the understanding of how m5C and its oxidative derivatives mediate their functions, we developed RNA baits bearing modified cytosines in diverse structural contexts to pulldown potential readers in C. elegans. Our mass spectrometry analyses reveal unique binding proteins for each of the modifications. We validate our dataset by demonstrating that the nematode ALYREF homologues ALY-1 and ALY-2 preferentially bind m5C in vitro. The dataset presented here serves as an important scientific resource that will support the discovery of new functions of m5C and its derivatives.
HostingRepository	PRIDE
AnnounceDate	2022-09-29
AnnouncementXML	Submission_2022-09-29_09:00:53.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Brenes
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 10:47:05	ID requested
⏵ 1	2022-09-29 09:00:54	announced

Dataset with its publication pending

submitter keyword: Caenorhabditis elegans, c elegans, label free

Angus I. Lamond
contact affiliation	Centre for Gene Regulation and Expression
contact email	a.i.lamond@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliation	University of Dundee
contact email	ajbrenesmurillo@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD035761

PRIDE project URI

• PRIDE
  1. PXD035761
     1. Label: PRIDE project
     2. Name: Identification of putative reader proteins of RNA 5-methylcytosine and its derivatives in Caenorhabditis elegans