PXD035712-1

PXD035712 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mitochondrial interactome quantitation reveals structural changes in metabolic machinery in the failing murine heart
Description	Advancements in cross-linking mass spectrometry (XL-MS) for structural analysis of proteins bridge the gap between purified systems and native tissue environments. Here, isobaric quantitative protein interaction reporter technology (iqPIR) was utilized to further extend XL-MS to the first system-wide comparative study of mitochondrial proteins from healthy and diseased murine hearts. The failing heart interactome includes 588 statistically significant cross-linked peptide pairs altered in the disease condition. Structural insight into ketone-oxidation metabolons, OXPHOS machinery, and nucleotide transporter hybrid-conformations support mitochondrial remodeling in failing hearts while bringing forth new hypotheses for pathological mechanisms. The application of quantitative cross-linking technology in tissue provides molecular-level insight into complex biological systems challenging to model in cell culture, thus providing a valuable resource for studying human diseases.
HostingRepository	PRIDE
AnnounceDate	2023-02-27
AnnouncementXML	Submission_2023-02-27_14:04:30.687.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AnnaBakhtina
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 01:43:25	ID requested
⏵ 1	2023-02-27 14:04:31	announced
2	2023-11-14 08:56:09	announced	2023-11-14: Updated project metadata.

Publication List

Keyword List

submitter keyword: quantitative cross-linking
heart failure
cardiac hypertrophy
mitochondria
protein interactions
interactome
mass spectrometry
systems structural biology

submitter keyword: quantitative cross-linking

heart failure

cardiac hypertrophy

mitochondria

protein interactions

interactome

mass spectrometry

systems structural biology

Contact List

James EdwardBruce
contact affiliation	University of Washington Department of Genome Sciences
contact email	jimbruce@uw.edu
lab head
AnnaBakhtina
contact affiliation	University of Washington
contact email	anna.bakhtina91@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035712
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035712

PRIDE project URI

Repository Record List

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