PXD035708-1

PXD035708 is an original dataset announced via ProteomeXchange.

Title	Loss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix.
Description	In this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in hTERT-BJ-5ta cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like TGF- β matrix regulation, epithelial mesenchymal transition (EMT), PI3K/AKT or P53. In conclusion, seems that the depletion of ALMS1 could be inhibiting the signals transduction through the TGF -β and the routes regulated downstream.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:27:20.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brais Bea Mascato
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 01:41:54	ID requested
⏵ 1	2024-01-26 06:27:21	announced
2	2024-10-22 06:25:33	announced	2024-10-22: Updated project metadata.

Bea-Mascato B, G, ó, mez-Casta, ñ, eda E, S, á, nchez-Corrales YE, Castellano S, Valverde D, Loss of the centrosomal protein ALMS1 alters lipid metabolism and the regulation of extracellular matrix-related processes. Biol Direct, 18(1):84(2023) [pubmed]

10.1186/s13062-023-00441-2;

submitter keyword: TGF-β, AKT, ciliopathy,ALMS1, Alström syndrome, LC-ESI-MS/MS, primary cilia

Brais Bea-Mascato
contact affiliation	University of Vigo
contact email	b.mascat@gmail.com
lab head
Brais Bea Mascato
contact affiliation	University of Vigo
contact email	b.mascat@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD035708

PRIDE project URI

• PRIDE
  1. PXD035708
     1. Label: PRIDE project
     2. Name: Loss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix.