PXD035698-1

PXD035698 is an original dataset announced via ProteomeXchange.

Title	Five-layer systems analysis of Leishmania stage differentiation reveals an essential role for protein degradation in parasite development
Description	Leishmania survival and pathogenicity depends on the parasite’s capacity to adapt to different host environments through stage differentiation of promastigotes within the sand fly, and of amastigotes inside mammalian host cells. Leishmania stage-specific expression occurs in the absence of classical transcriptional regulation, raising the question on alternative regulatory mechanisms. We investigated the role of protein turnover by applying label-free quantitative proteomic on both L. donovani amastigotes purified from the spleen of infected hamsters and the derived promastigotes in presence or absence of the irreversible, proteasomal inhibitor lactacystin. Proteins were prepared from four replicates for each condition.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-15_04:32:26.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Leishmania donovani; NCBI TaxID: NEWT:5661;
ModificationList	L-methionine (S)-sulfoxide; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 09:32:38	ID requested
⏵ 1	2026-06-15 04:32:26	announced

Dataset with its publication pending

submitter keyword: Label free,Leishmania donovani, proteasomal inhibitor, Q-Exactive Plus

Gerald, F, Spath
contact affiliation	Unité de Parasitologie moléculaire et Signalisation, Institut Pasteur, Université de Paris, INSERM U1201, Paris 75015, France
contact email	gerald.spaeth@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD035698

PRIDE project URI

• PRIDE
  1. PXD035698
     1. Label: PRIDE project
     2. Name: Five-layer systems analysis of Leishmania stage differentiation reveals an essential role for protein degradation in parasite development