PXD035669-3

PXD035669 is an original dataset announced via ProteomeXchange.

Title	A proteomic investigation of sporophyte and gametophyte in the red seaweed Asparagopsis
Description	The red seaweed, Asparagopsis taxiformis, is a promising ruminant feed-additive with significant anti-methanogenic properties, which could provide a global solution to climate-change. Emerging genomics in this species has provided a strong foundation for in-depth molecular investigations, including proteomics. Here, we investigated the A. taxiformis (L6) proteome of sporophyte and gametophyte (male and female), using soluble and insoluble extraction methods followed by mass spectrometry. A combined total of 741 and 2007 unique non-redundant proteins were identified using genome-derived and transcriptome-derived databases, respectively. Genome-derived proteins were used for further analysis including a relatively large proportion of proteins that were annotated as ion binding proteins (i.e. iron, zinc, manganese, potassium and copper), which may play a role in heavy metal bioremediation. In addition, we identified 44 photosynthesis-related proteins (e.g. phycobilisomes, photosystem I, photosystem II and ATPase) and 58 stress-related proteins (e.g. heat shock proteins and vanadium-dependent haloperoxidases). Forty proteins were predicted to be secreted, supporting a role for them in extracellular activities such as mucilage development and defence. Two Gal-2,6-Sulfurylases II proteins are likely necessary for carrageenan biosynthesis, as well as development of reproductive-associated structures. These findings provide a comprehensive overview of expressed proteins in A. taxiformis (L6) at two different life stages, highlighting the potential of proteomic approaches and warrant further studies for understanding protein functions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:41:49.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianfang Wang
SpeciesList	scientific name: Asparagopsis taxiformis; NCBI TaxID: 260499;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	X500R QTOF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 02:40:57	ID requested
1	2023-01-26 14:07:13	announced
2	2023-11-14 08:57:48	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:41:50	announced	2024-10-22: Updated project metadata.

10.3390/BIOLOGY12020167;

submitter keyword: LC-MS/MS, sporophyte, proteomics,Asparagopsis taxiformis (L6), gametophyte

Tianfang Wang
contact affiliation	Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia.
contact email	twang@usc.edu.au
lab head
Tianfang Wang
contact affiliation	University of the Sunshine Coast
contact email	twang@usc.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035669
     1. Label: PRIDE project
     2. Name: A proteomic investigation of sporophyte and gametophyte in the red seaweed Asparagopsis