PXD035650-2

PXD035650 is an original dataset announced via ProteomeXchange.

Title	Differential protein and glycan packaging into extracellular vesicles in response to 3D gastric cancer cellular organization
Description	Alterations of the glycosylation machinery are a common event in cancer that leads to the synthesis of aberrant glycan structures by tumor cells. Extracellular vesicles (EVs) play a modulator role in cancer communication and progression, and interestingly, several tumor-associated glycans have already been identified in cancer EVs. Nevertheless, the impact of 3D cellular architecture in the selective packaging of glycans into EVs has never been addressed. In this work, we have measured the capacity of gastric cancer cell lines carrying different glycosylation patterns in producing and releasing EVs when cultured under conventional 2D monolayer or in 3D culture conditions. Furthermore, we have identified the proteomic content and the presence of specific glycan patterns in the EVs produced by these cells, depending on their culture condition. We have observed that although the protein content of the analysed EVs was mostly conserved, an EV differential packaging of specific proteins and glycans was found, depending on the cell culture condition. Cellular component and pathway analysis have revealed different signatures on the EVs released by 2D and 3D cultured cells, suggesting distinct biological functions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:19.857.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035650
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 01:50:53	ID requested
1	2023-10-24 11:10:47	announced
⏵ 2	2023-11-14 09:07:22	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:08:46	announced	2024-10-22: Updated project metadata.

Martins Á M, Lopes TM, Diniz F, Pires J, Os, ó, rio H, Pinto F, Freitas D, Reis CA, Differential Protein and Glycan Packaging into Extracellular Vesicles in Response to 3D Gastric Cancer Cellular Organization. Adv Sci (Weinh), 10(24):e2300588(2023) [pubmed]

10.6019/PXD035650;

submitter keyword: glycosylation, 3D,EVs, gastric cancer

Celso Reis
contact affiliation	i3S-Institute for Research and Innovation in Health, University of Porto, Porto, Portugal
contact email	celsor@ipatimup.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035650
     1. Label: PRIDE project
     2. Name: Differential protein and glycan packaging into extracellular vesicles in response to 3D gastric cancer cellular organization