PXD035607-1

PXD035607 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Untargeted Metabolomics and Lipidomics Reveal Complex Pathways Spurred by Activation of Acid Resistance Mechanisms in Escherichia coli
Description	Escherichia coli (E. coli) is a ubiquitous group of bacteria that can be either commensal gut microbes or enterohemorrhagic food-borne pathogens. Regardless, both forms must survive acidic environments in the stomach and intestines to reach and colonize the gut, a process that partially relies on amino acid dependent acid resistance (AR) mechanisms and modifications to membrane phospholipids. However, only the basic tenets of these mechanisms have been elucidated. In this paper, we aim to conduct the first known full-scale metabolic and lipidomic characterization of E. coli adaptations to acid stress. We hypothesized that the use of untargeted metabolomics and lipidomics would reveal mechanisms downstream of AR processes that provide novel contributions to acid stress survival. We detected significant differences in the extracellular metabolome and the lipidome induced by amino acid supplementation (glutamine, arginine, or lysine) and contextualized these results using RT-qPCR. We additionally identified distinct metabolic and lipidomic pathways modulated by differential amino acid supplementation. These results demonstrate AR may not be simply a set of basic mechanisms but rather a coordinated acid resistant metabolic phenotype. Future studies may use our analysis to elucidate distinct targets for prebiotic supplements to cultivate commensal strains or therapies to combat pathogenic ones.
HostingRepository	MassIVE
AnnounceDate	2022-07-27
AnnouncementXML	Submission_2022-07-27_19:37:22.376.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Gold
SpeciesList	scientific name: Escherichia coli K-12; NCBI TaxID: 83333;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-27 18:47:56	ID requested
⏵ 1	2022-07-27 19:37:22	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: metabolomics, lipidomics, e coli, acid resistance

submitter keyword: metabolomics, lipidomics, e coli, acid resistance

Contact List

Andrew Gold
contact affiliation	The Ohio State University
contact email	gold.179@osu.edu
lab head
Andrew Gold
contact affiliation	The Ohio State University
contact email	gold.179@osu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089994/