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PXD035591-2

PXD035591 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructural surfaceomics of Nomo-1 using PhoX cross-linker with timsTOF
DescriptionThe project aimed to profile the cell surface proteins of Nomo-1 (AML cell line) using structural surfaceomics for identification of protein conformation-based cancer antigens thereby expanding the toolkit for cancer target discovery for immunotherapeutic targeting. To achieve the goal, cell surface capture (CSC) was integrated with cross-linking mass spectrometry (XL-MS). PhoX was used as a cross-linker to freeze the structural conformations of protein in three-dimensional space, followed by biotinylation of cell surface proteins to enable enrichment of cell surface proteins to allow focused XL-MS analysis of those enriched proteins. PhoX having in-built phosphonate-based IMAC handle which allowed additional enrichment of cross-linked peptides.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:04:58.268.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKamal Mandal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-25 11:30:25ID requested
12023-10-04 04:34:13announced
22024-10-22 06:04:58announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: XL-MS, PhoX, cell surfaceome,Nomo-1, AML
Contact List
Dr. Arun P. Wiita
contact affiliationDept. of Laboratory Medicine University of California, San Francisco, USA
contact emailarun.wiita@ucsf.edu
lab head
Kamal Mandal
contact affiliationUniversity of California, San Francisco
contact emailkamal.mandal@ucsf.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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