PXD035588 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Elucidating colony bloom formation mechanism of a harmful alga Phaeocystis globosa (Prymnesiophyceae) using metaproteomics |
Description | Phaeocystis is a globally distributed Prymnesiophyceae genus and usually forms massive harmful colony blooms, which impact marine ecosystem, mariculture, human health, and even threaten coastal nuclear power plant safety. However, the mechanisms behind the colony formation from the solitary cells remain poorly understood. Here, we investigated metabolic processes of both solitary and non-flagellated colonial cells of Phaeocystis globosa at different colonial bloom stages using a metaproteomic approach. Temperature was significantly correlated with Phaeocystis colony bloom formation, and the flagellated motile solitary cells with abundant flagellum-associated proteins, such as tubulin and dynein, were the exclusive cellular morphotype at the solitary cell stage featured with temperatures ≥ 21℃. When the temperature decreased to <21℃, tiny colonies appeared and the flagellum-associated proteins were identified lower abundances in both solitary and non-flagellated colonial cells, while proteins involved in biosynthesis, chain polymerization and aggregation of glycosaminoglycan (GAG), a key constituent of gelatinous matrix, were identified higher abundances, indicating the central role of active GAG biosynthesis during the colony formation. Furthermore, light utilization, carbon fixation, nitrogen assimilation, and amino acid and protein synthesis were also enhanced to provide sufficient energy and substrates for GAG biosynthesis. This study highlighted that temperature induced re-allocation of energy and substances toward GAG biosynthesis is essential for colony bloom formation of P. globosa. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:33:33.235.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Huamin Cheng |
SpeciesList | scientific name: Phaeocystis globosa; NCBI TaxID: 33658; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-25 11:29:25 | ID requested | |
1 | 2023-02-10 04:25:37 | announced | |
⏵ 2 | 2023-11-14 07:33:37 | announced | 2023-11-14: Updated project metadata. |
Publication List
Cheng HM, Zhang SF, Ning XL, Peng JX, Li DX, Zhang H, Zhang K, Lin L, Liu SQ, Smith WO, Wang DZ, Elucidating colony bloom formation mechanism of a harmful alga Phaeocystis globosa (Prymnesiophyceae) using metaproteomics. Sci Total Environ, 869():161846(2023) [pubmed] |
Keyword List
submitter keyword: Phaeocystis globosa |
Harmful colony bloom |
Colony formation |
Glycosaminoglycan biosynthesis |
Metaproteomics |
Contact List
Dazhi Wang |
contact affiliation | State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China |
contact email | dzwang@xmu.edu.cn |
lab head | |
Huamin Cheng |
contact affiliation | Xiamen University |
contact email | 1119091826@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035588
- Label: PRIDE project
- Name: Elucidating colony bloom formation mechanism of a harmful alga Phaeocystis globosa (Prymnesiophyceae) using metaproteomics