PXD035587-1

PXD035587 is an original dataset announced via ProteomeXchange.

Title	Unique Ligand and Kinase-Independent Roles of the Insulin Receptor in Regulation of Cell Cycle, Senescence and Apoptosis
Description	Insulin acts through the insulin receptor (IR) tyrosine kinase to exert its classical metabolic and mitogenic actions. Here, using receptors with either short or long deletion of the β-subunit or mutation of the kinase active site (K1030R, we uncover a second novel IR signaling pathway that is intracellular domain dependent, but ligand and tyrosine kinase-independent (LYK-I). These LYK-I actions of the IR are linked to changes in phosphorylation of a network of proteins involved in the regulation of extracellular matrix organization, cell cycle, ATM signaling and cellular senescence; and result in upregulation of expression of multiple extracellular matrix-related genes and proteins, down-regulation of immune/interferon-related genes and proteins, and increased sensitivity to apoptosis. Thus, in addition to classical ligand and tyrosine kinase-dependent (LYK-D) signaling, the IR regulates a second, novel ligand and tyrosine kinase-independent (LYK-I) pathway which regulates the cellular machinery involved in senescence, matrix interaction and response to extrinsic challenges.
HostingRepository	PRIDE
AnnounceDate	2023-01-02
AnnouncementXML	Submission_2023-01-02_08:25:26.471.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AshokkumarJayavelu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-25 11:27:07	ID requested
⏵ 1	2023-01-02 08:25:26	announced

Dataset with its publication pending

submitter keyword: Human,Kinase, Diabetes, Insulin Receptor,Insulin

Dr.Ashok KumarJayavelu
contact affiliation	Proteomics and Cancer Cell Signaling, Deutsches Krebsforschungszentrum,Heidelberg
contact email	ak.jayavelu@dkfz-heidelberg.de
lab head
AshokkumarJayavelu
contact affiliation	Dept Proteomics and Signal Transduction, MaxPlanck Institute of Biochemistry
contact email	jayavelu@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035587
     1. Label: PRIDE project
     2. Name: Unique Ligand and Kinase-Independent Roles of the Insulin Receptor in Regulation of Cell Cycle, Senescence and Apoptosis