PXD035569-1

PXD035569 is an original dataset announced via ProteomeXchange.

Title	UGO is a multipass membrane protein that is key to sense and transduce cues for schizont egress and gametocyte activation in malaria parasites
Description	Many critical events in the Plasmodium lifecycle depend on tight regulation of cGMP homeostasis by guanylyl cyclases (GC) and phosphodiesterases including egress of schizonts from the host erythrocyte and activation of gametocytes upon a mosquito blood-feed. Both processes are triggered by distinct cues but similarly depend on GCα for cGMP production. How these cues are integrated by the same pathway remains a mystery. Here we show that in gametocytes, temperature-dependent epistatic interactions between phosphodiesterases prevent activation prior to a blood-feed. We demonstrate that GCα interacts with two multipass membrane cofactors, UGO and SLF, in schizonts and gametocytes. While SLF is important to regulate GCα basal activity, UGO is essential for schizont egress and gametocyte activation but not for GCα basal activity. Instead, UGO is key to upregulate GCα in response to signals that lower the tension of parasite membranes, including the mosquito molecule xanthurenic acid or a rise in extracellular pH. Altogether our results suggest that intramembrane sensing of distinct signals by UGO represents a focal point to enhance GCα activity and trigger timely schizont egress and activation of gametogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-04-28
AnnouncementXML	Submission_2023-04-28_05:02:42.187.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035569
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	alexandreHainard
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-25 02:13:35	ID requested
⏵ 1	2023-04-28 05:02:42	announced

10.6019/PXD035569;

submitter keyword: LC-MSMS, gametocyte, immunoprecipitation,plasmodium berghei

MathieuBrochet
contact affiliation	Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
contact email	mathieu.brochet@unige.ch
lab head
alexandreHainard
contact affiliation	University of Geneva
contact email	alexandre.hainard@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035569
     1. Label: PRIDE project
     2. Name: UGO is a multipass membrane protein that is key to sense and transduce cues for schizont egress and gametocyte activation in malaria parasites