PXD035550-1

PXD035550 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CellEKT: A chemical proteomics assay to profile cellular target engagement of kinase inhibitors
Description	Activity-based protein profiling is a powerful tool to study target engagement in complex biological environments. XO44 is a chemical probe based on the scaffold of a promiscuous kinase binder, which covalently reacts with a conserved lysine in the ATP-binding pocket of kinases. Pretreatment of cells with a drug (candidate) results in competition of XO44 binding, which can be interpreted as in situ target engagement. Here, a workflow is presented which is optimized for kinome coverage, reproducibility and data processing. This dataset pertains to the selectivity testing of BTK inhibitors in MV4-11 cells.
HostingRepository	PRIDE
AnnounceDate	2025-04-08
AnnouncementXML	Submission_2025-04-08_07:19:43.070.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Rüegger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-24 05:23:59	ID requested
⏵ 1	2025-04-08 07:19:43	announced

Publication List

10.1016/J.MCPRO.2025.100961;

10.1016/J.MCPRO.2025.100961;

Keyword List

submitter keyword: drug discovery, chemical proteomics, kinase,ABPP

submitter keyword: drug discovery, chemical proteomics, kinase,ABPP

Contact List

Mario van
contact affiliation	Professor of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Joel Rüegger
contact affiliation	Leiden University
contact email	j.ruegger@lic.leidenuniv.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD035550
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD035550

PRIDE project URI

Repository Record List

[ + ]