PXD035532-1

PXD035532 is an original dataset announced via ProteomeXchange.

Title	A metabolic map of the DNA damage response identifies PRDX1 in nuclear ROS scavenging and aspartate synthesis
Description	While cellular metabolism impacts the DNA damage response, a systematic understanding of the metabolic requirements that are crucial for DNA damage repair has yet to be achieved. Here, we investigate the metabolic enzymes and processes that are essential when cells are exposed to DNA damage. By integrating functional genomics with chromatin proteomics and metabolomics, we provide a detailed description of the interplay between cellular metabolism and the DNA damage response. Subsequent analysis identified Peroxiredoxin 1, PRDX1, as fundamental for DNA damage repair. During the DNA damage response, PRDX1 translocates to the nucleus where it is required to reduce DNA damage-induced nuclear reactive oxygen species levels. Moreover, PRDX1 controls aspartate availability, which is required for the DNA damage repair-induced upregulation of de novo nucleotide synthesis. Loss of PRDX1 leads to an impairment in the clearance of γΗ2ΑΧ nuclear foci, accumulation of replicative stress and cell proliferation defects, thus revealing a crucial role for PRDX1 as a DNA damage surveillance factor.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_11:07:18.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frédéric FONTAINE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-24 04:59:37	ID requested
⏵ 1	2024-10-07 11:07:18	announced

Dataset with its publication pending

submitter keyword: DNA damage response

Peroxiredoxin 1

reactive oxygen species scavenging

electron transport chain

chromatin

aspartate metabolism

Sara Sdelci
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology; Gene Regulation, Stem Cells and Cancer; Sdelci Lab; Barcelona, Spain
contact email	sara.sdelci@crg.eu
lab head
Frédéric FONTAINE
contact affiliation	CeMM Research Center for Molecular Medicine
contact email	frederic.fontaine.pro@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035532
     1. Label: PRIDE project
     2. Name: A metabolic map of the DNA damage response identifies PRDX1 in nuclear ROS scavenging and aspartate synthesis