PXD035478-1

PXD035478 is an original dataset announced via ProteomeXchange.

Title	Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization
Description	The development of a prophylactic vaccine for Kaposi sarcoma-associated Herpesvirus (KSHV) would prevent consequences from infection including disorders such as Kaposi sarcoma and primary effusion lymphoma. Here, we study the immunogenicity of noninfectious virus-like vesicles (VLVs) of KSHV as a potential future vaccine platform. VLVs present a repertoire of viral structural proteins but are noninfectious due to a defect in capsid formation that prevents viral DNA packaging. Immunization of mice with adjuvanted VLVs results in virus-specific antibodies and T cells. These antibodies neutralize viral infection, and this neutralization is enhanced by the complement system. Complement-enhanced neutralization is dependent on antibodies targeting the SCR region of viral ORF4. However, this activity was not present in serum from KSHV-infected humans. Our study highlights an important role of antibody effector functions in the development of a future KSHV vaccine
HostingRepository	PRIDE
AnnounceDate	2023-02-01
AnnouncementXML	Submission_2023-02-01_10:37:18.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JeffreyJohnson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human gammaherpesvirus 8; NCBI TaxID: NCBITaxon:37296;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-21 03:40:15	ID requested
⏵ 1	2023-02-01 10:37:19	announced
2	2024-10-22 05:41:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: KSHV herpesvirus

JeffreyJohnson
contact affiliation	microbiology department at the Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
lab head
JeffreyJohnson
contact affiliation	Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035478
     1. Label: PRIDE project
     2. Name: Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization