PXD035433-1

PXD035433 is an original dataset announced via ProteomeXchange.

Title	Targeted cross-linker delivery for in situ mapping of protein conformations and interactions in mitochondria
Description	Current methods for intracellular protein analysis mostly require the separation of specific organelles or the change of intracellular environment. However, the functions of these proteins are determined by their native microenvironment as they usually form complexes with ions, nucleic acids, and other proteins. Thus, we have explored a method for in situ crosslinking and mapping of mitochondrial proteins in living cells. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles are functionalized with dihexadecyldimethylammonium bromide (DDAB) to deliver protein crosslinkers into mitochondria. In situ crosslinked proteins are analyzed using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-05-26
AnnouncementXML	Submission_2023-05-26_01:14:50.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YuwanChen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 03:43:12	ID requested
⏵ 1	2023-05-26 01:14:51	announced

Dataset with its publication pending

submitter keyword: Mitochondria, In-situ cross-linking, Nanoparticles delivery, Protein conformations and interactions,Chemical cross-linking mass spectrometry

LihuaZhang
contact affiliation	CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
contact email	lihuazhang@dicp.ac.cn
lab head
YuwanChen
contact affiliation	CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
contact email	zpcyw@dicp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD035433

PRIDE project URI

• PRIDE
  1. PXD035433
     1. Label: PRIDE project
     2. Name: Targeted cross-linker delivery for in situ mapping of protein conformations and interactions in mitochondria