PXD035426-2

PXD035426 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mapping diversity in African trypanosomes using high resolution spatial proteomics
Description	African trypanosomes are dixenous eukaryotic parasites that impose a significant human and veterinary disease burden on sub-Saharan Africa. Diversity between species and life-cycle stages is concomitant with distinct host and tissue tropisms within this group. Here, the spatial proteomes of two African trypanosome species, Trypanosoma brucei and Trypanosoma congolense, have been mapped, each in mammalian and insect life-stages represented by bloodstream form (BSF) and procyclic form (PCF) respectively. Using the hyperLOPIT (hyperplexed localisation of organelle proteins by isotope tagging) methodology, this work has provided four highly comprehensive spatial proteomes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:15.155.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035426
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicola Moloney
SpeciesList	scientific name: Trypanosoma congolense; NCBI TaxID: 5692; scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 01:23:34	ID requested
1	2023-10-22 16:09:37	announced
⏵ 2	2023-11-14 09:06:16	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD035426;
Moloney NM, Barylyuk K, Tromer E, Crook OM, Breckels LM, Lilley KS, Waller RF, MacGregor P, Mapping diversity in African trypanosomes using high resolution spatial proteomics. Nat Commun, 14(1):4401(2023) [pubmed]

10.6019/PXD035426;

Moloney NM, Barylyuk K, Tromer E, Crook OM, Breckels LM, Lilley KS, Waller RF, MacGregor P, Mapping diversity in African trypanosomes using high resolution spatial proteomics. Nat Commun, 14(1):4401(2023) [pubmed]

Keyword List

submitter keyword: Trypanosoma brucei, hyperLOPIT,African trypanosomes, spatial proteome, parasite diversification, speciation, Trypanosoma congolense, protein localisation

submitter keyword: Trypanosoma brucei, hyperLOPIT,African trypanosomes, spatial proteome, parasite diversification, speciation, Trypanosoma congolense, protein localisation

Contact List

Paula MacGregor
contact affiliation	School of Biological Sciences, University of Bristol
contact email	paula.macgregor@bristol.ac.uk
lab head
Nicola Moloney
contact affiliation	University of Cambridge
contact email	nm657@cam.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD035426
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD035426

PRIDE project URI

Repository Record List

[ + ]