PXD035418-1

PXD035418 is an original dataset announced via ProteomeXchange.

Title	Regulation and physiological function of proteins for heat tolerance in cowpea (Vigna unguiculata) genotypes under controlled and field conditions
Description	The expression of heat shock proteins is considered a central adaptive mechanism to heat stress. This study investigated the expression of heat shock proteins (HSPs) and other stress-protective proteins against heat stress in cowpea genotypes under field (IT-96D-610 and IT-16) and controlled (IT-96D-610) conditions. Plants in the control environment were under 25/20°C (day/night) in a warm chamber and 40/25 °C in a hot chamber. In the field, plants grew at Eensaamheid with 26 to 28°C and at Marapyane with 30 to 32°C monthly average maximum temperatures. Heat stress response analysis of proteins at 72 h in the controlled environment showed 270 differentially regulated proteins identified using label-free quantitative proteomics in IT-96D-610 plants. These plants expressed HSPs and chaperones (BAG family molecular chaperone 6 (BAG6), Multiprotein bridging factor1c (MBF1C) and cold shock domain protein 1 (CSDP1)) in the controlled environment. However, IT-96D-610 plants expressed a wider variety of small HSPs and more HSPs in the field. IT-96D-610 plants also responded to heat stress by exclusively expressing chaperones (DnaJ chaperones, universal stress protein and heat shock binding protein (HSBP)) and non-HSP proteins (Deg1, EGY3, ROS protective proteins, temperature-induced lipocalin and succinic dehydrogenase). Photosynthesis recovery and induction of proteins related to photosynthesis, were better in IT-96D-610 because of the concurrent induction of heat stress response proteins for chaperone functions, protein degradation for repair and ROS scavenging proteins and PSII operating efficiency (Fq’/Fm’) than IT-16. This study contributes to identification of thermotolerance mechanisms in cowpea that can be useful in knowledge-based crop improvement.
HostingRepository	PRIDE
AnnounceDate	2022-08-08
AnnouncementXML	Submission_2022-08-08_11:26:31.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tonny Selinga
SpeciesList	scientific name: Vigna unguiculata subsp. unguiculata; NCBI TaxID: 3920;
ModificationList	No PTMs are included in the dataset
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 01:06:42	ID requested
⏵ 1	2022-08-08 11:26:31	announced
2	2023-11-14 08:57:36	announced	2023-11-14: Updated project metadata.

10.3389/FPLS.2022.954527;

submitter keyword: LC-MS, label-free protein quantification

Samson Chimphango
contact affiliation	Department of Biology, Plant ecophysiology lab, ADepartment of Biological Sciences, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa
contact email	samson.chimphango@uct.ac.za
lab head
Tonny Selinga
contact affiliation	University of Cape Town
contact email	selingati@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035418
     1. Label: PRIDE project
     2. Name: Regulation and physiological function of proteins for heat tolerance in cowpea (Vigna unguiculata) genotypes under controlled and field conditions