PXD035372-1

PXD035372 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of ORM2 overexpression in the liver of ob/ob mice
Description	In order to evaluate ORM2 could activate which signaling pathway, phosphoproteomic analysis was performed. First, liver proteins were extracted from ob/ob mice injected with Ad-ORM2 and Ad-GFP using RIPA lysis buffer containing protease inhibitors and phosphatase inhibitors. Next, the phosphorylated peptides were enriched by immobilized metal affinity chromatograph and the fractions were pre-separated by reversed phase liquid chromatograph. Electron transfer dissociation was used to effectively retain the skeletal information and phosphorylation modification information of the peptide, and accurately determine the molecular mass of proteins or peptides. Finally, the phosphoproteomic analysis was performed by tandem mass spectrometry and database retrieval.
HostingRepository	iProX
AnnounceDate	2022-07-15
AnnouncementXML	Submission_2022-07-17_20:03:40.478.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Lu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-17 20:03:34	ID requested
⏵ 1	2022-07-17 20:03:40	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomic analysis, Liver, Mice, ORM2

Yan Lu
contact affiliation	Zhongshan Hospital, Fudan University
contact email	rjluyan@126.com
lab head
Yan Lu
contact affiliation	Zhongshan Hospital, Fudan University
contact email	rjluyan@126.com
dataset submitter

iProX dataset URI