PXD035365-1

PXD035365 is an original dataset announced via ProteomeXchange.

Title	Disrupting metformin adaptation of hepatocellular carcinoma by targeting TOMM34/ATP5B axis
Description	As a well-known anti-diabetic drug, metformin has been repurposed for cancer treatment, but drug resistance and tumor metastasis observed recently have challenged its further application. Here, we found that long-term metformin exposure led to metabolic adaptation of HCC cells, which was characterized by obvious epithelial-mesenchymal transition (EMT) phenotype and compensatory elevated oxidative phosphorylation (OXPHOS). TOMM34, a translocase of the outer mitochondrial membrane, was identified to confer energetic flexibility thus promoting tumor metastasis in response to metformin-induced metabolic stress. Mechanistically, TOMM34 interacted and stabilized ATP5B to preserve F1F0-ATPase activity, which promoted mitochondrial OXPHOS and increased cellular ATP levels. This metabolic preference to OXPHOS suggested a large demand of energy supply of cancer cells to survival and spread under therapeutic stress.
HostingRepository	iProX
AnnounceDate	2022-07-14
AnnouncementXML	Submission_2022-07-17_19:38:25.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ping Jin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-17 19:38:06	ID requested
⏵ 1	2022-07-17 19:38:26	announced
2	2022-12-24 20:54:31	announced	2022-12-25: Update publication information.

Dataset with its publication pending

submitter keyword: Metformin, Metabolic adaptation, Hepatocellular carcinoma, Metastasis, OXPHOS

Canhua Huang
contact affiliation	Sichuan University
contact email	1665705251@qq.com
lab head
Ping Jin
contact affiliation	Sichuan University
contact email	654504559@qq.com
dataset submitter

iProX dataset URI