PXD035312-1
PXD035312 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Vitamin C modulates the levels of several proteins of the mitochondrial complex III and its activity in the mouse liver |
| Description | In the present study, we used the Gulo-/- female and male mice model which depends entirely on ascorbate derived from the diet. Importantly, the levels of ascorbate found in the serum of Gulo-/- mice reflect the amounts of ascorbate provided in drinking water. We tested different concentrations of ascorbate ranging from 0 to 0.4% (w/v) ascorbate, added into drinking water, until the age of four months. We performed label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) global quantitative proteomic profiling to identify and quantify proteins in microsomal enriched liver extracts (MEE) from our different ascorbate treated cohorts of Gulo-/- females and males. We found that the MS intensities of several proteins in the mitochondrial complex III of the electron transport chain correlated positively with liver ascorbate concentrations in both Gulo-/- females and males. Consequently, the mitochondrial complex III activity in Gulo-/- female and male mice treated with suboptimal hepatic concentrations of ascorbate was significantly lower than Gulo-/- mice treated with optimal ascorbate concentration. Finally, proteins involved in complement activation correlated negatively with liver ascorbate concentrations in both Gulo-/- males and females. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-09-27 |
| AnnouncementXML | Submission_2022-09-27_08:58:42.831.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michel Lebel |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-07-13 10:55:36 | ID requested | |
| ⏵ 1 | 2022-09-27 08:58:43 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Vitamin C, gulonolactone oxidase, mass spectrometry, mitochondrial complex III, reactive oxygen species, sex-related differences, liver, mouse |
Contact List
| Michel Lebel | |
|---|---|
| contact affiliation | Centre Hospitalier Universite Laval |
| contact email | michel.lebel@crchudequebec.ulaval.ca |
| lab head | |
| Michel Lebel | |
| contact affiliation | CHudeQuebe |
| contact email | michel.lebel@crchudequebec.ulaval.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089881/ |
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