PXD035269 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CD11b restricts alveolar macrophage activation during pneumococcal pneumonia and prevents excessive lung inflammation |
Description | Bacterial lung infections are associated with strong infiltration of CD11b+ myeloid cells, which limit life-threatening disease, but also severely damage lung tissue. In a murine lung infection model with Streptococcus pneumoniae, we found intrinsic upregulation of CD11b on resident alveolar macrophages. Such CD11b expression was associated with transcriptomic and proteomic adaptations by alveolar macrophages, leading to the identification of specific molecules and pathways that depended on CD11b. In the absence of CD11b, the antimicrobial defense of alveolar macrophages was strongly reduced, and the production of neutrophil-recruiting chemokines was more pronounced. Moreover, CD11b expression limited the infection and prevented excessive alveolar damage. In conclusion, our study provides detailed molecular insights into the alveolar macrophage-specific immune response to Streptococcus pneumoniae lung infection and reveals profound CD11b-dependent alterations that are critical for effective antimicrobial immunity, neutrophil recruitment, and prevention of alveolar damage. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:05:41.636.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thilo Bracht |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-11 15:05:32 | ID requested | |
1 | 2023-10-08 08:27:04 | announced | |
⏵ 2 | 2023-11-14 09:05:49 | announced | 2023-11-14: Updated project metadata. |
Publication List
Zec K, Thiebes S, Bottek J, Siemes D, Spangenberg P, Trieu DV, Kirstein N, Subramaniam N, Christ R, Klein D, Jendrossek V, Loose M, Wagenlehner F, Jablonska J, Bracht T, Sitek B, Budeus B, Klein-Hitpass L, Theegarten D, Shevchuk O, Engel DR, Comparative transcriptomic and proteomic signature of lung alveolar macrophages reveals the integrin CD11b as a regulatory hub during pneumococcal pneumonia infection. Front Immunol, 14():1227191(2023) [pubmed] |
Keyword List
submitter keyword: Streptococcus pneumoniae,Alveolar Macrophages, Integrin α-M |
Contact List
Thilo Bracht |
contact affiliation | Medizinisches Proteom-Center, Ruhr-University Bochum and Department of Anesthesia, Intensive Care Medicine and Pain Therapy, University Hospital Knappschaftskrankenhaus Bochum |
contact email | thilo.bracht@rub.de |
lab head | |
Thilo Bracht |
contact affiliation | Clinical Proteomics |
contact email | thilo.bracht@rub.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035269
- Label: PRIDE project
- Name: CD11b restricts alveolar macrophage activation during pneumococcal pneumonia and prevents excessive lung inflammation