PXD035261 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tumor decellularization reveals proteomic and mechanical characteristics of the extracellular matrix of primary liver cancer |
Description | Tumor initiation and progression are critically dependent on interaction of cancer cells with their cellular and extracellular microenvironment. Alterations in the composition, integrity, and mechanical properties of the extracellular matrix (ECM) dictate tumor processes including proliferation, migration, and invasion. Also in primary liver cancers, consisting of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), the dysregulation of the extracellular environment by liver fibrosis and tumor desmoplasia is pertinent. Yet, in-depth characterization of liver cancer ECM and underlying tumor-promoting mechanisms remain largely unknown. Herein, we used an integrative molecular and mechanical approach to extensively characterize the ECM of HCC and CCA tumors by utilizing decellularization techniques. We identified a myriad of ECM-related proteins in both tumor and adjacent liver tissue, highlighting the complexity of the primary liver cancer matrisome. The differences in ECM protein abundance result in divergent mechanical properties on a macro- and micro-scale that is tumor-type specific. Furthermore, we employed the decellularized tumor ECM to create a tumor-specific hydrogel that support patient-derived tumor organoids. This provides a new avenue for personalized medicine by combining patient-derived tumor ECM and cancer cells. Taken together, this study provides better understanding of alterations to key aspects of the ECM that occur during primary liver cancer development. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-28 |
AnnouncementXML | Submission_2023-03-28_08:10:33.750.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JeroenDemmers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-11 13:56:06 | ID requested | |
⏵ 1 | 2023-03-28 08:10:34 | announced | |
Publication List
van Tienderen GS, Conboy J, Muntz I, Willemse J, Tieleman J, Monfils K, Schurink IJ, Demmers JAA, Doukas M, Koenderink GH, van der Laan LJW, Verstegen MMA, Tumor decellularization reveals proteomic and mechanical characteristics of the extracellular matrix of primary liver cancer. Biomater Adv, 146():213289(2023) [pubmed] |
Keyword List
submitter keyword: decellularization |
extracellular matrix |
primary liver cancer |
hepatocellular carcinoma |
cholangiocarcinoma |
Contact List
JeroenDemmers |
contact affiliation | Erasmus MC |
contact email | j.demmers@erasmusmc.nl |
lab head | |
JeroenDemmers |
contact affiliation | Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands |
contact email | j.demmers@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035261
- Label: PRIDE project
- Name: Tumor decellularization reveals proteomic and mechanical characteristics of the extracellular matrix of primary liver cancer