PXD035234-1

PXD035234 is an original dataset announced via ProteomeXchange.

Title	Endometrial protein expression and phosphorylation landscape decipher aberrant insulin and mTOR signaling in patients with recurrent pregnancy loss
Description	Research question: What are the proteomic and phosphoproteomic differences between the endometrium of women with recurrent pregnancy loss (RPL) and healthy control women during the proliferative (P) and secretory (S) phases of the menstrual cycle? Design: The present study collected a total of 54 endometrial samples during either P or S phases from women with RPL (n = 28) or healthy control (n = 26). Comprehensive proteomic and phosphoproteomic analyses were conducted using label-free liquid chromatography-tandem mass spectrometry (n = 44) and verified through western blotting (n = 10). Three comparison groups were established, including the total RPL endometrium compared to the total control (R/C), proliferating endometrium compared to control (RP/CP), and secretory endometrium compared to control (RS/CS). Results: Differentially expressed proteins (DEPs) and differentially phosphorylated proteins (DPPs) were respectively identified in the three comparison groups. Combining pathway enrichment, network analysis, and soft clustering analysis, we identified the insulin/cyclic nucleotide signaling pathway and AMPK/mTOR signaling pathway as the major contributors to the aberration of RPL endometrium. Western blotting verified altered expression of four proteins, including PRKAR1B, ADCY3, PRKAA2, and LPIN2. Conclusions: This exploratory study provides insights into the differentiated protein expression and phosphorylation profiles of RPL endometrium in both P and S phases. The results highlight potential proteins associated with RPL pathogenesis that may serve as potential indicators for RPL. The findings contribute to the identification of potential targets for RPL treatment as well as the pathogenesis of RPL.
HostingRepository	PRIDE
AnnounceDate	2023-10-27
AnnouncementXML	Submission_2023-10-26_21:17:49.200.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JingHang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 08:23:33	ID requested
⏵ 1	2023-10-26 21:17:49	announced
2	2023-11-14 08:18:34	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:24	announced	2024-10-22: Updated project metadata.

submitter keyword: Phosphoproteomics, Cyclic nucleotide signaling, Endometrium, Proteomics, AMPK/mTOR signaling,RPL

JingHang
contact affiliation	Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China;
contact email	hangjbysy@163.com
lab head
JingHang
contact affiliation	Peking University Third Hospital
contact email	hangjbysy@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035234
     1. Label: PRIDE project
     2. Name: Endometrial protein expression and phosphorylation landscape decipher aberrant insulin and mTOR signaling in patients with recurrent pregnancy loss