PXD035212-1

PXD035212 is an original dataset announced via ProteomeXchange.

Title	Identification of PP1c-PPP1R12A Substrates Using K-BIPS (Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates)
Description	Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) interacts with the catalytic subunit of protein phosphatase 1 (PP1c) to form one of the many protein phosphatase 1 complexes, PP1c-PPP1R12A (or myosin phosphatase). In addition to a well-documented role in muscle contraction, the PP1c-PPP1R12A complex is associated with cytoskeleton organization, cell migration, adhesion, cell division, and insulin signaling. Despite the variety of biological functions, only a few substrates of the PP1c-PPP1R12A complex are characterized, which limits a full understanding of PP1c-PPP1R12A activities. Here, the chemoproteomics method K-BIPS (Kinase-catalyzed Biotinylation to Identify Phosphatase Substrates) was used to identify substrates of the PP1c-PPP1R12A complex in L6 skeletal muscle cells. K-BIPS enriched 136 candidate substrates with 14 high confidence hits. Two high confidence hits, AKT1 kinase and COPS4 signalosome proteins, were studied as novel PP1c-PPP1R12A substrates. Interestingly, both AKT1 and COPS4 were previously documented to regulate PP1c-PPP1R12A phosphatase activity. Therefore, the data suggest that PP1c-PPP1R12A influences its own phosphatase activity through substrate-dependent feedback mechanisms
HostingRepository	PRIDE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_10:51:24.449.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035212
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	EricDavis
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 02:39:05	ID requested
⏵ 1	2023-10-24 10:51:25	announced
2	2023-11-14 09:06:50	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:08:13	announced	2024-10-22: Updated project metadata.

Dedigama-Arachchige PM, Acharige NPN, Zhang X, Bremer HJ, Yi Z, Pflum MKH, Identification of PP1c-PPP1R12A Substrates Using Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates. ACS Omega, 8(39):35628-35637(2023) [pubmed]

10.6019/PXD035212;

submitter keyword: Protein Phosphatase 1

PP1

PPP1R12A

chemoproteomics

substrate identification

Mary KPflum
contact affiliation	Wayne State University Chemistry
contact email	pflum@wayne.edu
lab head
EricDavis
contact affiliation	Wayne State University
contact email	gm9453@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035212
     1. Label: PRIDE project
     2. Name: Identification of PP1c-PPP1R12A Substrates Using K-BIPS (Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates)