PXD035199-2

PXD035199 is an original dataset announced via ProteomeXchange.

Title	Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics
Description	Extracellular vesicle (EV) analysis from blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health and disease. Technical variation must be minimized to confidently assess EV-associated biomarkers but the impact of pre-analytics on EV characteristics in blood samples remains minimally explored. We present the results from the first large-scale EV Blood Benchmarking (EVBB) study in which we systematically compared 11 blood collection tubes (BCT; 6 preserving and 5 non-preserving) and 3 blood processing intervals (BPI; 1h, 8h and 72h) on defined performance metrics (n=9). The EVBB study identifies a significant impact of multiple BCT and BPI on a diverse set of metrics reflecting blood sample quality, ex-vivo generation of blood-cell derived EV, EV recovery and EV-associated molecular signatures. The results assist the informed selection of the optimal BCT and BPI for EV analysis. The proposed metrics serve as a platform to guide future research on EV pre-analytics and further support methodological standardization of EV studies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:36.127.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pekka Rappu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-09 09:04:43	ID requested
1	2023-03-13 00:12:11	announced
⏵ 2	2023-11-14 08:57:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Biomarkers

Proteomics

Extracellular Vesicles

Exosomes

Serum

Plasma

Anticoagulants

Preservatives

Cancer

Olivier De Wever
contact affiliation	Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium
contact email	Olivier.DeWever@UGent.be
lab head
Pekka Rappu
contact affiliation	University of Turku
contact email	pekka.rappu@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035199

PRIDE project URI

• PRIDE
  1. PXD035199
     1. Label: PRIDE project
     2. Name: Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics