PXD035166-1

PXD035166 is an original dataset announced via ProteomeXchange.

Title	Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W
Description	Protein post-translational modification regulates near every aspect of cellular processes in eukaryotes. However, the identification of new protein post-translational modification is very challenging. Here, using genetically encoded unnatural amino acids as chemical probes, we report the identification and validation of a previously unreported form of protein post-translational modification, aminoacylated lysine ubiquitination, in which the modification occurs on the α-amine group of aminoacylated lysine. We identify more than two thousands of unique aminoacylated lysine ubiquitination sites on all 20 aminoacylated lysines in two human cell lines. The aminoacylated lysine ubiquitination modifications can mediate rapid protein degradation, complementing the canonical lysine ubiquitination-mediated proteome degradation. Furthermore, we demonstrate that the ubiquitin conjugating enzyme UBE2W acts as a writer of aminoacylated lysine ubiquitination and facilitates the ubiquitination of internal α-amine groups on proteins using an unexpected enzymatic activity. More broadly, the discovery and validation of aminoacylated lysine ubiquitination paves the way for the identification and verification of new protein post-translation modifications with the genetic code expansion strategy.
HostingRepository	iProX
AnnounceDate	2022-07-20
AnnouncementXML	Submission_2022-07-19_19:31:54.570.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yulin Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-07 18:59:19	ID requested
⏵ 1	2022-07-19 19:31:54	announced

Dataset with its publication pending

submitter keyword: aminoacylated lysine ubiquitination, GCE

Shixian Lin
contact affiliation	Zhejiang University
contact email	sxlin@zju.edu.cn
lab head
Yulin Chen
contact affiliation	Zhejiang University
contact email	ychen209@qq.com
dataset submitter

iProX dataset URI