PXD035110-2

PXD035110 is an original dataset announced via ProteomeXchange.

Title	Vap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase
Description	To identify Vap33 binding proteins, we purified Vap33-containing protein complexes from Drosophila embryos, using the single-step GFP-trap bead purification from a fly line expressing YFP-tagged Vap33, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of Vap33 to RtGEF and Git, which we further connected to the regulation of Hippo signaling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:21.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexey Veraksa
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:12:16	ID requested
1	2024-02-01 12:12:02	announced
⏵ 2	2024-10-22 06:28:22	announced	2024-10-22: Updated project metadata.

10.1242/jcs.261917;

Portela M, Mukherjee S, Paul S, La Marca JE, Parsons LM, Veraksa A, Richardson HE, The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism. J Cell Sci, 137(4):(2024) [pubmed]

submitter keyword: Vap33, embryo,Drosophila

Alexey Veraksa
contact affiliation	Biology Department University of Massachusetts Boston Boston, USA
contact email	alexey.veraksa@umb.edu
lab head
Alexey Veraksa
contact affiliation	University of Massachusetts Boston
contact email	alexey.veraksa@umb.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD035110

PRIDE project URI

• PRIDE
  1. PXD035110
     1. Label: PRIDE project
     2. Name: Vap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase