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PXD035110 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase
DescriptionTo identify Vap33 binding proteins, we purified Vap33-containing protein complexes from Drosophila embryos, using the single-step GFP-trap bead purification from a fly line expressing YFP-tagged Vap33, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of Vap33 to RtGEF and Git, which we further connected to the regulation of Hippo signaling.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexey Veraksa
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListiodoacetic acid derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-06 14:12:16ID requested
12024-02-01 12:12:02announced
Publication List
Portela M, Mukherjee S, Paul S, La Marca JE, Parsons LM, Veraksa A, Richardson HE, The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism. J Cell Sci, 137(4):(2024) [pubmed]
Keyword List
submitter keyword: Vap33, embryo,Drosophila
Contact List
Alexey Veraksa
contact affiliationBiology Department University of Massachusetts Boston Boston, USA
contact emailalexey.veraksa@umb.edu
lab head
Alexey Veraksa
contact affiliationUniversity of Massachusetts Boston
contact emailalexey.veraksa@umb.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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