PXD035109-2

PXD035109 is an original dataset announced via ProteomeXchange.

Title	The Holdup Multiplex, an assay for high-throughput measurement of protein-ligand affinity constants using a mass-spectrometry readout
Description	We developed the Holdup Multiplex, which quantifies hundreds of protein-peptide affinities at unprecedented accuracy with only one single experiment, using a label-free quantitative mass-spectrometry strategy. We applied this strategy to the seven human 14 3 3 proteins, and depicted new rules for 14 3 3 binding and specificity, valid at the proteome scale. Our approach is readily transferable to affinity-quantify the interactome of any protein of interest for any pool of ligands.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:41:29.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francois Delalande
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:11:56	ID requested
1	2022-12-20 21:24:24	announced
⏵ 2	2024-10-22 05:41:30	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: 14-3-3, AP-MS, DIA, phosphorylation

Christine Carapito
contact affiliation	CNRS, UMR7178, LSMBO, 67087 Strasbourg, FRANCE Université de Strasbourg, IPHC, 25 rue Becquerel, 67087 Strasbourg, FRANCE
contact email	ccarapito@unistra.fr
lab head
Francois Delalande
contact affiliation	CNRS
contact email	delaland@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD035109

PRIDE project URI

• PRIDE
  1. PXD035109
     1. Label: PRIDE project
     2. Name: The Holdup Multiplex, an assay for high-throughput measurement of protein-ligand affinity constants using a mass-spectrometry readout