PXD035107-1

PXD035107 is an original dataset announced via ProteomeXchange.

Title	Global phosphoproteomic profiling of skeletal muscle in ovarian-hormone deficient mice
Description	Phosphorylation is important in skeletal muscle development, growth, regeneration, and contractile function. Alterations in the skeletal muscle phosphoproteome due to aging have been reported in males; however, studies in females are lacking. We have demonstrated that estrogen deficiency decreases muscle force which correlates with decreased myosin regulatory light chain phosphorylation. Thus, we questioned whether the decline of estrogen in females that occurs with aging might alter the skeletal muscle phosphoproteome. C57BL/6J female mice randomly assigned to a sham-operated (Sham) or ovariectomy (Ovx) group to investigate the effects of estrogen deficiency on skeletal muscle protein phosphorylation in a resting, non-contracting condition. After 16 weeks of estrogen deficiency, the tibialis anterior muscle was dissected and prepped for label-free nano-liquid chromatography tandem mass spectrometry phosphoproteomic analysis. We identified 4,780 phosphopeptides in tibialis anterior muscles of ovariectomized (Ovx) and Sham-operated (Sham) control mice. Further analysis revealed 647 differentially regulated phosphopeptides (Benjamini – Hochberg adjusted p-value < 0.05 and 1.5-fold change ratio) that corresponded to 130 proteins with 22 proteins differentially phosphorylated (3 unique to Ovx, two unique to Sham, six upregulated, and 11 downregulated). Differentially phosphorylated proteins associated with the sarcomere, cytoplasm, and metabolic and calcium signaling pathways were identified. Our work provides the first global phosphoproteomic analysis in females and how estrogen deficiency impacts the skeletal muscle phosphoproteome.
HostingRepository	PRIDE
AnnounceDate	2022-09-28
AnnouncementXML	Submission_2022-09-28_13:29:37.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mina Peyton
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:11:02	ID requested
⏵ 1	2022-09-28 13:29:38	announced
2	2023-11-14 08:56:44	announced	2023-11-14: Updated project metadata.

10.1152/PHYSIOLGENOMICS.00104.2022;

submitter keyword: females, estrogen, ovariectomy, sarcomere, tropomyosin, obscurin, myosin heavy chain-2, myozenin-2, and calcium signaling

Dawn A. Lowe
contact affiliation	University of Minnesota - Rehabilitation Science
contact email	lowex017@umn.edu
lab head
Mina Peyton
contact affiliation	University of MN
contact email	mina.peyton@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035107
     1. Label: PRIDE project
     2. Name: Global phosphoproteomic profiling of skeletal muscle in ovarian-hormone deficient mice