PXD035105-1

PXD035105 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of peroxisome-associated PEX5 mutants in Xenopus egg extract.
Description	This project sought to identify the features in PEX5 required for interaction with the peroxisomal PEX2-PEX10-PEX12 ubiquitin ligase complex. Peroxisomes equilibrated with different PEX5 mutants (i.e., C11A, AH1, AH2) were purified from Xenopus egg extract, solubilized in digitonin, and PEX5-associated material was immunoprecipitated via a C-terminal FLAG tag. PEX5 without a FLAG tag was used as a negative control. PEX5-interacting peroxins were identified by LC-MS/MS at the Taplin Mass Spectrometry Facility at Harvard Medical School.
HostingRepository	PRIDE
AnnounceDate	2022-08-16
AnnouncementXML	Submission_2022-08-15_17:42:54.613.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035105
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Skowyra
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:10:21	ID requested
⏵ 1	2022-08-15 17:42:55	announced

Skowyra ML, Rapoport TA, PEX5 translocation into and out of peroxisomes drives matrix protein import. Mol Cell, 82(17):3209-3225.e7(2022) [pubmed]

submitter keyword: PEX5, peroxisome

Tom Abraham Rapoport
contact affiliation	Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
contact email	Tom_Rapoport@hms.harvard.edu
lab head
Michael Skowyra
contact affiliation	Harvard Medical School
contact email	michael_skowyra@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD035105

PRIDE project URI

• PRIDE
  1. PXD035105
     1. Label: PRIDE project
     2. Name: Proteomic analysis of peroxisome-associated PEX5 mutants in Xenopus egg extract.