PXD035083-1

PXD035083 is an original dataset announced via ProteomeXchange.

Title	Proteomic responses of two spring wheat cultivars to combined drought and aphid Metopolophium dirhodum) infestation
Description	In the field, plants usually have to face combined effects of abiotic and biotic stresses. In our study, two spring wheat cultivars Septima and Quintus were subjected to three water regimes (70, 50, 40 % soil water capacity SWC), aphid (Metopolophium dirhodum) infestation or the combination of both stresses, i.e., drought (50, 40% SWC) and aphids. The study has a 2x3x2 factorial design with three biological replicates. Our previous study (Saska et al. 2022) investigated plant morphophysiological characteristics and aphid life table. In the present study, the results of proteomic analysis using 2D-DIGE followed by MALDI-TOF/TOF protein identification are presented. Drought, but also aphid infestation led to alterations in 113 protein spots including proteins assigned to a variety of biological processes ranging from signalling via energy metabolism, redox regulation, stress and defense responses to secondary metabolism indicating a long-term adaptation to adverse conditions. The absence of specific proteins involved in plant response to herbivorous insects indicates a loss of resistance to aphids in modern wheat cultivars during the breeding process. Septima revealed enhanced tolerance with respect to Quintus as indicated by higher values of morphophysiological characteristics (above-ground biomass, leaf length, osmotic potential per full water saturation) and rel. abundance of proteins involved in mitochondrial respiration and ATP biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:36:34.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Planchon
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	L-kynurenine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-04 15:01:19	ID requested
⏵ 1	2024-10-08 11:36:34	announced

Dataset with its publication pending

submitter keyword: spring wheat

drought

herbivorous insects

combined stress

proteome

2D-DIGE

Pavel Vítámvás
contact affiliation	Crop Research Institute, Drnovska 507/73, 161 01 Prague, Czech Republic
contact email	vitamvas@vurv.cz
lab head
Sebastien Planchon
contact affiliation	Luxembourg Institute of Science and Technology
contact email	sebastien.planchon@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035083
     1. Label: PRIDE project
     2. Name: Proteomic responses of two spring wheat cultivars to combined drought and aphid Metopolophium dirhodum) infestation