PXD035078-2

PXD035078 is an original dataset announced via ProteomeXchange.

Title	Evaluation of absorbent cotton for glycopeptide enrichment
Description	This study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the performance of cotton HILIC was evaluated by comparing with other two commercial enrichment approaches, venusil HILIC and Oasis MAX in both mouse brain and seminal plasma. Moreover, capacity and recovery rate were evaluated using different milligram of cotton in 200µL tips. Thus, a simple, convenient, and cost-friendly cotton HILIC method was proposed to achieve highly selective intact glycopeptide profiling.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:07.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miaomiao Xin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-04 14:58:55	ID requested
1	2023-03-04 01:44:54	announced
⏵ 2	2023-11-14 08:56:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: HILIC,Glycopeptide, Enrichment Absorbent cotton, Mass spectrometry, MAX

Shisheng Sun
contact affiliation	Northwest University
contact email	suns@nwu.edu.cn
lab head
Miaomiao Xin
contact affiliation	College of Life Science, Northwest University, Xi’an, Shaanxi Province 710069, P. R. China
contact email	xinmiao1206@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035078

PRIDE project URI

• PRIDE
  1. PXD035078
     1. Label: PRIDE project
     2. Name: Evaluation of absorbent cotton for glycopeptide enrichment