PXD035050 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The regulatory landscape of the yeast phosphoproteome – quantitative phosphoproteomic DIA data |
Description | We systematically exposed the yeast Saccharomyces cerevisiae to a wide array of 101 perturbations and generated a quantitative mass spectrometry-based atlas of early phosphoproteomic responses. Perturbations covered broad classes of environmental, chemical, and drug perturbations and systematically targeted diverse aspects of yeast cell biology. Importantly, most perturbations focused on capturing early signaling responses (5 min treatments), with the reasoning that functional phosphorylation of specific substrates occurs rapidly following stimulation, but promiscuous phosphorylation and confounding changes in transcript and protein abundances takes place more slowly. Treatments were done in 6 biological replicates and large-scale phosphoproteomics was enabled by our recently developed R2-P2 (Rapid-Robotic Phosphoproteomics) sample preparation workflow in combination with DIA measurements. This submission includes 559 quantitative phospho DIA-MS raw files that went into the analysis of the systems-level phosphorylation stress response. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:02:34.033.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Leutert |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-30 07:48:07 | ID requested | |
1 | 2023-09-04 05:41:05 | announced | |
⏵ 2 | 2024-10-22 06:02:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Signaling, DIA,Phosphoproteomics, Orbitrap Exploris 480, PTM, Saccharomyces cerevisiae, Phosphatase, Budding Yeast, R2-P2, Stress Response, Kinase, Phosphorylation |
Contact List
Judit Villén |
contact affiliation | Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA |
contact email | jvillen@uw.edu |
lab head | |
Mario Leutert |
contact affiliation | Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA |
contact email | marioleutert@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD035050 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035050
- Label: PRIDE project
- Name: The regulatory landscape of the yeast phosphoproteome – quantitative phosphoproteomic DIA data