PXD035030-2

PXD035030 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis of platelet glycoprotein Ibα glycosylation.
Description	Background: Platelet glycoprotein (GP) Ibα is the major ligand-binding subunit of the GPIb-IX-V complex that binds von Willebrand Factor (VWF). GPIbα is heavily glycosylated, and its glycans have been proposed to play key roles in platelet clearance, VWF binding, and as target antigens in immune thrombocytopenia syndromes. Despite its importance in platelet biology, the glycosylation profile of GPIbα is not well characterized. Objectives: The aim of this study was to comprehensively analyze GPIbα amino acid sites of glycosylation (glycosites) and glycan structures. Methods: GPIbα ectodomain that was recombinantly expressed or that was purified from human platelets was analyzed by Western blot, mass spectrometry (MS) glycomics, and MS glycoproteomics to define glycosites and the structures of the attached glycans. Results: We identified a diverse repertoire of N- and O-glycans, including sialoglycans, Tn antigen, T antigen, and ABH blood group antigens. In the analysis of the recombinant protein, we identified 62 unique O-glycosites. In the analysis of the endogenous protein purified from platelets, we identified at least 48 unique O-glycosites and 1 N-glycosite. The GPIbα mucin domain is densely O-glycosylated. Glycosites are also located within the macroglycopeptide domain and mechanosensory domain (MSD). Conclusions: This comprehensive analysis of GPIbα glycosylation lays the foundation for further studies to determine the functional and structural roles of GPIbα glycans.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:18.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie Hollenhorst
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 07:18:10	ID requested
1	2023-02-09 08:24:35	announced
⏵ 2	2023-11-14 08:57:19	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, platelets, glycoproteins

Stacy A Malaker
contact affiliation	Yale Department of Chemistry
contact email	stacy.malaker@yale.edu
lab head
Marie Hollenhorst
contact affiliation	Brigham and Women's Hospital, Division of Hematology, Harvard Medical School
contact email	mhollenhorst@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035030
     1. Label: PRIDE project
     2. Name: Comprehensive analysis of platelet glycoprotein Ibα glycosylation.