PXD034986-1

PXD034986 is an original dataset announced via ProteomeXchange.

Title	RNA-DNA hybrids prevent resection at dysfunctional telomeres
Description	At critically short telomeres TERRA RNA-DNA hybrids become stabilized and drive homology-directed repair (HDR) to delay replicative senescence. However, even at long- and intermediate-length telomeres, not subject to HDR, transient TERRA RNA-DNA hybrids form, suggestive of additional roles. Here, we report that hybrids at telomeres prevent resection by the Exo1 nuclease when telomeres become non-functional. We employed the well-characterized cdc13-1 allele, where telomere resection can be induced in a temperature dependent manner, to demonstrate that ssDNA generation at telomeres is either prevented or augmented when RNA-DNA hybrids are stabilized or destabilized, respectively. The viability of cdc13-1 cells is affected by the presence or absence of hybrids accordingly. These results give insights into an additional role of TERRA at dysfunctional telomeres suggesting that it not only affects replicative senescence rates through HDR activation at critically short telomeres, but may also affect resection rates at intermediate length telomeres in pre-senescent cells.
HostingRepository	PRIDE
AnnounceDate	2023-01-18
AnnouncementXML	Submission_2023-01-18_03:51:29.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FButter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 02:55:59	ID requested
⏵ 1	2023-01-18 03:51:29	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS

FalkButter
contact affiliation	Institute of Molecular Biology (IMB)
contact email	f.butter@imb.de
lab head
FButter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034986
     1. Label: PRIDE project
     2. Name: RNA-DNA hybrids prevent resection at dysfunctional telomeres