PXD034964-1

PXD034964 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins that interact with TRAF6 in ADP-heptose- or IL-1β-stimulated HEK293 cells
Description	The bacterial metabolite ADP-heptose activates the mammalian protein kinase ALPK1 (alpha protein kinase 1), which then phosphorylates TIFA (TRAF interacting protein with FHA domain). This leads to the oligomerisation of TIFA and the recruitment and oligomerisation of TRAF6 (TNF receptor-associated factor 6), activating a signalling network that culminates in the production of inflammatory mediators that mount responses to combat microbial infection. To identify other protein(s) that associate with TRAF6 in ADP-heptose-stimulated cells, we re-expressed FLAG-TRAF6 in TRAF6 KO cells, stimulated the cells with ADP-heptose and immunoprecipitated TRAF6 from the cell extracts using a FLAG antibody. Proteins associated with TRAF6 were identified by mass spectrometry and included the components of LUBAC (the linear ubiquitin assembly complex), the components of the TAK1 and IB kinase (IKK) complexes, TRAF2 and c-IAP1, providing insight into the molecular details and mode of operation of the ADP-heptose signalling pathway. In parallel experiments the cells were stimulated with IL-1b, a cytokine that also signals via TRAF6, which served as a control.
HostingRepository	PRIDE
AnnounceDate	2022-09-14
AnnouncementXML	Submission_2022-09-14_01:36:36.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederic Lamoliatte
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-29 15:07:20	ID requested
⏵ 1	2022-09-14 01:36:37	announced
2	2023-11-14 08:57:35	announced	2023-11-14: Updated project metadata.

10.1042/BCJ20220401;

submitter keyword: ALPK1, TIFA, ADP-heptose, TRAF6

Professor Sir Philip Cohen
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, The Sir James Black Centre, University of Dundee, United Kingdom
contact email	p.cohen@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliation	University of Dundee
contact email	flamoliatte001@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD034964

PRIDE project URI

• PRIDE
  1. PXD034964
     1. Label: PRIDE project
     2. Name: Identification of proteins that interact with TRAF6 in ADP-heptose- or IL-1β-stimulated HEK293 cells