PXD034935-1

PXD034935 is an original dataset announced via ProteomeXchange.

Title	Identification of LRP1 as novel interactor of 3-O sulfated heparan sulfates via proteome profiling of human CSF
Description	Heparan (HS) proteoglycans (HSPGs) are common on the cell surface and mediate many physiological processes. Binding of HSPG ligands is determined by the sulfation code on the heparan sulfate chain and the HS chain can be N-/2-O/6-O- or 3-O-sulfated, generating a heterogenous sulfation pattern. 3-O sulfated HS (3S-HS) have been reported to play a role in several pathological processes such as the coagulation system, viral pathogenesis, or in the binding and internalization of tau in Alzheimer’s disease. Few 3S-HS-specific interactors are known and the role of 3S-HS in health and disease is limited, especially in the central nervous system. Using human CSF as a surrogate for the extracellular compartment of the CNS, we determined the interactome of synthetic heparan sulfate oligosaccharides with defined sulfation patterns. These AE-MS studies significantly expand the proteins that can interact with HS and for which 3O sulfation is required. LRP1 was found as a novel 3S-HS interactor, requiring GlcA-GlcNS6S3S for binding, similar to ATIII. Both interactions were validated via Western blot. Our dataset brings forward new HS and 3S-HS ligands which can be pertinent to unravel molecular mechanisms dependent on 3S-HS in (patho)physiological conditions.
HostingRepository	PRIDE
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2022-08-09_09:15:12.950.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034935
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	An Staes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-27 02:47:43	ID requested
⏵ 1	2022-08-09 09:15:13	announced

Dataset with its publication pending

submitter keyword: HS-protein interaction

HS 3-O sulfation interactome

heparan sulfates

alzheimer’s disease;

Kris Gevaert
contact affiliation	VIB-UGent Center for Medical Biotechnology Gent Department of Biomolecular Medicine
contact email	kris.gevaert@vib-ugent.be
lab head
An Staes
contact affiliation	Medical Protein Chemistry
contact email	an.staes@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034935
     1. Label: PRIDE project
     2. Name: Identification of LRP1 as novel interactor of 3-O sulfated heparan sulfates via proteome profiling of human CSF